Cryopreservation of plant cell cultures (in Japanese)



















  • Sakai A, Kobayashi S, Oiyama I (1991) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb.) by simple freezing method. Plant Science 74: 243–248. DOI: 10.1016/0168-9452(91)90052-A: https://dx.doi.org/10.1016/0168-9452(91)90052-A

  • Menges M, Murray JAH (2004) Cryopreservation of transformed and wild-type Arabidopsis and tobacco cell suspension cultures. Plant Journal 37: 635–644. DOI: 10.1046/j.1365-313X.2003.01980.x: https://dx.doi.org/10.1046/j.1365-313X.2003.01980.x

  • Kobayashi T, Niino T, Kobayashi M (2005) Simple cryopreservation protocol with an encapsulation technique for tobacco BY-2 suspension cell cultures. Plant Biotechnology 22: 105–112. DOI: 10.5511/plantbiotechnology.22.105: https://dx.doi.org/10.5511/plantbiotechnology.22.105

  • Kobayashi T, Niino T, Kobayashi M (2006) Cryopreservation of tobacco BY-2 suspension cell cultures by vitrification with encapsulation. Plant Biotechnology 23: 333–338. DOI: 10.5511/plantbiotechnology.23.333: https://dx.doi.org/10.5511/plantbiotechnology.23.333

  • Ogawa Y, Suzuki H, Sakurai N, Aoki K, Saito K, Shibata D (2008) Cryopreservation and metabolicprofiling analysis of Arabidopsis T87 suspension-cultured cells. Cryo-Letters 29: 427–436.: https://www.ingentaconnect.com/content/cryo/cryo/2008/00000029/00000005/art00008

  • Ogawa Y, Sakurai N, Oikawa A, Kai K, Morishita Y, Mori K, Moriya K, Fujii F, Aoki K, Suzuki H, Ohta D, Saito K, Shibata D (2012) High-throughput cryopreservation of plant cell cultures for functional genomics. Plant and Cell Physiology 53: 943–952. DOI: 10.1093/pcp/pcs038: https://dx.doi.org/10.1093/pcp/pcs038











Technical training course at RIKEN BioResource Research Center: “Cryopreservation of tobacco BY-2 cells” (September 12, 2022)