======================================================================================================= medium no. 7: Linsmaier and Skoog medium, 1 µM 2,4-dichlorophenoxyacetic acid, 1.2% (w/v) agar, pH 5.7 ======================================================================================================= Plant cell lines ================= * :doc:`/cell_lines/rpc00010` * :doc:`/cell_lines/rpc00011` * :doc:`/cell_lines/rpc00021` Chemicals and stock solutions ============================== (All stock solutions are stored at 4°C) * MS salt mix Murashige and Skoog Plant Salt Mixture, FUJIFILM Wako Pure Chemical Corporation (#392-00591) * Sucrose * LS_VT .. csv-table:: :header: "Chemical", "Concentration (mg/mL)" :widths: auto "Thiamine·HCl", "0.16" "*myo*-Inositol", "40" * 2,4-D (1 mM) .. csv-table:: :header: "Chemical", "Concentration (mg/mL)" :widths: auto "2,4-D sodium monohydrate", "0.261" (2,4-Dichlorophenoxy)acetic acid sodium salt monohydrate, Sigma-Aldrich (D6679) * Agar, powder * KOH (1 N) Medium preparation =================== #. Dissolve the following chemicals in approximately 800 mL of distilled water. .. csv-table:: :header: "Chemical", "Amount" :widths: auto "MS salt mix", "1 bag (1 L)" "Sucrose", "30 g" #. Add following stock solutions, and fill up to approximately 950 mL with distilled water. .. csv-table:: :header: "Stock solution", "Volume (mL)" :widths: auto "LS_VT", "2.5" "2,4-D (1 mM)", "1" #. Adjust the pH of the solution to 5.7 with KOH (1 N), and fill up to 1 L with distilled water. #. Add 12 g of agar to the medium. #. Autoclave the medium at 121°C for 20 min. Formulation ============ .. csv-table:: :header: "Chemical", "Concentration (mg/L)" :widths: auto "KNO\ :sub:`3`", "1900" "NH\ :sub:`4`\ NO\ :sub:`3`", "1650" "CaCl\ :sub:`2`\ ·2H\ :sub:`2`\ O", "440" "MgSO\ :sub:`4`\ ·7H\ :sub:`2`\ O", "370" "KH\ :sub:`2`\ PO\ :sub:`4`", "170" "H\ :sub:`3`\ BO\ :sub:`3`", "6.2" "MnSO\ :sub:`4`\ ·4H\ :sub:`2`\ O", "22.3" "ZnSO\ :sub:`4`\ ·7H\ :sub:`2`\ O", "8.6" "KI", "0.83" "Na\ :sub:`2`\ MoO\ :sub:`4`\ ·2H\ :sub:`2`\ O", "0.25" "CuSO\ :sub:`4`\ ·5H\ :sub:`2`\ O", "0.025" "CoCl\ :sub:`2`\ ·6H\ :sub:`2`\ O", "0.025" "FeSO\ :sub:`4`\ ·7H\ :sub:`2`\ O", "27.8" "Na\ :sub:`2`\ -EDTA", "37.3" "Thiamine·HCl", "0.4" "*myo*-Inositol", "100" "Sucrose", "30000" "2,4-D sodium monohydrate", "0.261" "Agar", "12000"