=============================================================================== medium no. 60: 1/2 modified Murashige and Skoog medium, 1 mg/L kinetin, pH 5.8 =============================================================================== Plant cell lines ================= * :doc:`/cell_lines/rpc00100` Chemicals and stock solutions ============================== (All stock solutions are stored at 4°C) * MS salt mix Murashige and Skoog Plant Salt Mixture, FUJIFILM Wako Pure Chemical Corporation (#392-00591) * Sucrose * MS_VT .. csv-table:: :header: "Chemical", "Concentration (mg/mL)" :widths: auto "Nicotinic acid", "0.5" "Pyridoxine·HCl", "0.5" "Thiamine·HCl", "0.1" "Glycine", "2" * MS_inositol .. csv-table:: :header: "Chemical", "Concentration (mg/mL)" :widths: auto "*myo*-Inositol", "40" * Kinetin (0.2 mg/mL) .. csv-table:: :header: "Chemical", "Concentration (mg/mL)" :widths: auto "Kinetin", "0.2" Dissolve kinetin in small volume of KOH (1 N), and fill up with distilled water * KOH (1 N) Medium preparation =================== #. Dissolve the following chemicals in approximately 800 mL of distilled water. .. csv-table:: :header: "Chemical", "Amount" :widths: auto "MS salt mix", "0.5 bag (equivalent with 0.5 L)" "Sucrose", "30 g" #. Add following stock solutions, and fill up to approximately 950 mL with distilled water. .. csv-table:: :header: "Stock solution", "Volume (mL)" :widths: auto "MS_VT", "0.5" "MS_inositol", "1.25" "Kinetin (0.2 mg/mL)", "5" #. Adjust the pH of the solution to 5.8 with KOH (1 N), and fill up to 1 L with distilled water. #. Autoclave the medium at 121°C for 20 min. Formulation ============ .. csv-table:: :header: "Chemical", "Concentration (mg/L)" :widths: auto "KNO\ :sub:`3`", "950" "NH\ :sub:`4`\ NO\ :sub:`3`", "825" "CaCl\ :sub:`2`\ ·2H\ :sub:`2`\ O", "220" "MgSO\ :sub:`4`\ ·7H\ :sub:`2`\ O", "185" "KH\ :sub:`2`\ PO\ :sub:`4`", "85" "H\ :sub:`3`\ BO\ :sub:`3`", "3.1" "MnSO\ :sub:`4`\ ·4H\ :sub:`2`\ O", "11.15" "ZnSO\ :sub:`4`\ ·7H\ :sub:`2`\ O", "4.3" "KI", "0.415" "Na\ :sub:`2`\ MoO\ :sub:`4`\ ·2H\ :sub:`2`\ O", "0.125" "CuSO\ :sub:`4`\ ·5H\ :sub:`2`\ O", "0.0125" "CoCl\ :sub:`2`\ ·6H\ :sub:`2`\ O", "0.0125" "FeSO\ :sub:`4`\ ·7H\ :sub:`2`\ O", "13.9" "Na\ :sub:`2`\ -EDTA", "18.65" "Nicotinic acid", "0.25" "Pyridoxine·HCl", "0.25" "Thiamine·HCl", "0.05" "Glycine", "1" "*myo*-Inositol", "50" "Sucrose", "30000" "Kinetin", "1"